Neuroimunology is a field which combines neuroscience and immunology. Mass cytometry, or CyTOF (cytometry by time-of-flight), is a technique applied to the discovery of novel immune populations in humans and rodents. It uses metal-conjugated antibodies to enable a high-dimensional description of tens of markers at the single-cell level, thereby providing a bird's-eye view of the immune system. Brain perfusion, extraction of the brain tissue and its dissociation into a single-cell suspension, followed by cell staining with metal-tagged antibodies, sample reading using a mass cytometer, and data analysis using SPADE and viSNE are the diffrent stages involved in the mass cytometry approach. Sepsis is a serious infection in which a patient die as aresult of organ failure due to the correlation of systemic and uncontrolled immune activation. The pathogenesis of hashimoto encephalopathy is not clear, mainly because of the lacking of exact neuropathologic data .
- Track 1-1 Mass cytometry analysis
- Track 2-2 Stepsis biomarkers
- Track 3-3 Hashimoto encephalopathy
- Track 4-4 Hippocampus neuroinflamatory response